Genomic and cDNA Sequence Tags of the Hyperthermophilic Archaeon Pyrobaculum Aerophilum
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چکیده
منابع مشابه
Genomic and cDNA sequence tags of the hyperthermophilic archaeon Pyrobaculum aerophilum.
The hyperthermophilic archaeum, Pyrobaculum aerophilum, grows optimally at 100 degrees C with a doubling time of 180 min. It is a member of the phylogenetically ancient Thermoproteales order, but differs significantly from all other members by its facultatively aerobic metabolism. Due to its simple cultivation requirements and its nearly 100% plating efficiency, it was chosen as a model organis...
متن کاملGenome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
We determined and annotated the complete 2.2-megabase genome sequence of Pyrobaculum aerophilum, a facultatively aerobic nitrate-reducing hyperthermophilic (T(opt) = 100 degrees C) crenarchaeon. Clues were found suggesting explanations of the organism's surprising intolerance to sulfur, which may aid in the development of methods for genetic studies of the organism. Many interesting features wo...
متن کاملEffect of tungstate on nitrate reduction by the hyperthermophilic archaeon pyrobaculum aerophilum
Pyrobaculum aerophilum, a hyperthermophilic archaeon, can respire either with low amounts of oxygen or anaerobically with nitrate as the electron acceptor. Under anaerobic growth conditions, nitrate is reduced via the denitrification pathway to molecular nitrogen. This study demonstrates that P. aerophilum requires the metal oxyanion WO42- for its anaerobic growth on yeast extract, peptone, and...
متن کاملProperties of a thermostable nitrate reductase from the hyperthermophilic archaeon Pyrobaculum aerophilum.
The nitrate reductase of the hyperthermophilic archaeon Pyrobaculum aerophilum was purified 137-fold from the cytoplasmic membrane. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, the enzyme complex consists of three subunits with apparent molecular weights of 130,000, 52,000, and 32,000. The enzyme contained molybdenum (0.8-mol/mol complex), iron (15.4-mol/mol comp...
متن کاملnitrate - reducing hyperthermophilic archaeum . Pyrobaculum aerophilum sp . nov
A novel rod-shaped hyperthermophilic archaeum has been isolated from a boiling marine water hole at Maronti Beach, Ischia, Italy. It grew optimally at 100°C and pH 7.0 by aerobic respiration as well as by dissimilatory nitrate reduction, forming dinitrogen as a final product. Organic and inorganic compounds served as substrates during aerobic and anaerobic respiration. Growth was inhibited by e...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.22.4373